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- Title
Dieckol isolated from Eisenia bicyclis extract suppresses RANKL-induced osteoclastogenesis in murine RAW 264.7 cells.
- Authors
Su-Hyeon Cho; Tae-Hyung Kwon; Hoibin Jeong; Jin Sook Kim; Song-Rae Kim; Myeong Seon Jeong; SeonJu Park; Miri Choi; Jung-Hee Woo; Juhee Ahn; Kil-Nam Kim
- Abstract
Objective: To demonstrate the effect of dieckol from Eisenia bicyclis on osteoclastogenesis using RAW 264.7 cells. Methods: Murine macrophage RAW 264.7 cells were subjected to dieckol treatment, followed by treatment with receptor activator of nuclear factor kappa-B ligand (RANKL) to induce osteoclastogenesis. Tartrate-resistant acid phosphatase (TRAP) activity was examined using a TRAP activity kit. Western blotting analysis was conducted to examine the level of osteoclast-related factors, including TRAP and calcitonin receptor (CTR), transcriptional factors, including c-Fos, c-Jun, and nuclear factor of activated T cells cytoplasmic 1 (NFATc1), nuclear factor kappa-B (NF-κB), extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK). Immunofluorescence staining was conducted to examine the expression of c-Fos, c-Jun, and NFATc1. Results: Among the four phlorotannin compounds present in Eisenia bicyclis, dieckol significantly hindered osteoclast differentiation and expression of RANKL-induced TRAP and CTR. In addition, dieckol downregulated the expression levels of c-Fos, c-Jun, NFATc1, ERK, and JNK, and suppressed NF-κB signaling. Conclusions: Dieckol can suppress RANKL-induced osteoclastogenesis. Therefore, it has therapeutic potential in treating osteoclastogenesis-associated diseases.
- Subjects
TRANCE protein; OSTEOCLASTOGENESIS; CALCITONIN receptors
- Publication
Asian Pacific Journal of Tropical Biomedicine, 2022, Vol 12, Issue 6, p262
- ISSN
2221-1691
- Publication type
Article
- DOI
10.4103/2221-1691.345518