We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Estandarización de la técnica para la obtención de trypomastigotes en células 3T3 a partir de una cepa local de epimastigotes de Tripanosoma cruzi.
- Authors
Córdova Rojas, N. Marisol; Torrico, Mary Cruz; Torrico, Faustino; Suárez Barrientos, Eduardo L.
- Abstract
Objective: to evaluate the use of cell cultures for the production of trypomastigotes in 3T3 cells, from a local strain of Trypanosoma cruzi epimastigotes. Method: we previously performed growing 3T3 cells in DMEM-10% FBS more penicillin-streptomycin in vitro at 37 °C, 95% humidity and 5% CO2 on the seventh day, they were infected with T. cruzi epimastigotes TcV strain, isolated from patients with acute Chagas and preliminarily grown in biphasic media as NNN and LIT. Results: after 14 days of infection was observed the parasite forms: extracellular) that were released into the infected cells once lysed. Subsequent sub 3T3 cell cultures trypomastigotes obtained from epimastigotes obtaining improved T. cruzi-TcV. Conclusions: it is possible to obtain trypomastigotes from a local strain epimastigotes recreating the life cycle of the parasite in vitro.
- Publication
Gaceta Médica Boliviana, 2015, Vol 38, Issue 1, p6
- ISSN
1012-2966
- Publication type
Article