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- Title
Microarray analysis refines classification of non-medullary thyroid tumours of uncertain malignancy.
- Authors
Fontaine, J.-F.; Mirebeau-Prunier, D.; Franc, B.; Triau, S.; Rodien, P.; Houlgatte, R.; Malthièry, Y.; Savagner, F.
- Abstract
Conventional histology failed to classify part of non-medullary thyroid lesions as either benign or malignant. The group of tumours of uncertain malignancy (T-UM) concerns either atypical follicular adenomas or the recently called ‘tumours of uncertain malignant potential’. To refine this classification we analysed microarray data from 93 follicular thyroid tumours: 10 T-UM, 3 follicular carcinomas, 13 papillary thyroid carcinomas and 67 follicular adenomas, compared to 73 control thyroid tissue samples. The diagnosis potential of 16 selected genes was validated by real-time quantitative RT–PCR on 6 additional T-UM. The gene expression profiles in several groups were examined with reference to the mutational status of the RET/PTC, BRAF and RAS genes. A pathological score (histological and immunohistochemical) was estimate for each of the T-UM involved in the study. The correlation between the T-UM gene profiles and the pathological score allowed a separation of the samples in two groups of benign or malignant tumours. Our analysis confirms the heterogeneity of T-UM and highlighted the molecular similarities between some cases and true carcinomas. We demonstrated the ability of few marker genes to serve as diagnosis tools and the need of a T-UM pathological scoring.Oncogene (2008) 27, 2228–2236; doi:10.1038/sj.onc.1210853; published online 29 October 2007
- Subjects
TUMORS; THYROID diseases; HETEROGENEITY; RAS oncogenes; GENE expression; GENETIC regulation; PATHOLOGY
- Publication
Oncogene, 2008, Vol 27, Issue 15, p2228
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1210853