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- Title
Protection of blue light induced retinal degeneration by the free radical scavenger Phenyl‐N‐tert‐butylnitrone and a serotonin receptor 5‐HT1a agonist in rats.
- Authors
GRILLO‐ANTONELLI, S; CIMBOLINI, N; DUBOS, H; FERAILLE, L; MARGARON, P; ELENA, P
- Abstract
Purpose Blue light exposition of rat retina induces retinal damages that mimic age‐related macular degeneration (AMD), showing severe photoreceptor cell death, apoptosis of RPE cells, and progressive outer nuclear layer destruction due to reactive oxygen species, and therefore providing an useful model to test potential AMD treatments. Here we proposed to compare the protective effects of two compounds, the antioxidant free radical scavenger, Phenyl‐N‐tert‐butylnitrone (PBN) and the serotonin receptor 5‐HT1a agonist 8‐Hydroxy‐2‐(di‐n‐propylamino)tetralin, 8‐OH‐DPAT, on photo‐oxidative stress in rat retina. Methods After a period of dark adaptation, Sprague‐Dawley rats were exposed to blue light during 6 hours. Animals were divided in three groups: 50 mg/kg PBN injected intraperitoneally on the day of induction, 500µg/kg 8‐OH‐DPAT or NaCl 0.9% subcutaneously injected once a day, starting 2 days before light exposure. Retinal function and anatomical integrity were evaluated by scotopic electroretinography (ERG) and optical coherence tomography, respectively, 1 and 2 weeks after induction. Outer nuclear layer thickness was measured on histological sections harvested 2 weeks after the induction. Results Significant protection of retina from degeneration was achieved with both compounds, which effectively rescued a wave and b wave amplitudes of ERG responses and preserved photoreceptor layer thickness. Conclusion The serotonin receptor 5‐HT1a could be a valuable target for AMD and other retinal degenerative diseases. Commercial interest
- Subjects
SEROTONIN agonists; FREE radical scavengers; RETINAL degeneration; BLUE light; MACULAR degeneration
- Publication
Acta Ophthalmologica (1755375X), 2012, Vol 90, p0
- ISSN
1755-375X
- Publication type
Article
- DOI
10.1111/j.1755-3768.2012.S063.x