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- Title
Laminin chains in rat and human peripheral nerve: Distribution and regulation during development and after axonal injury.
- Authors
Wallquist, Wilhelm; Patarroyo, Manuel; Thams, Sebastian; Carlstedt, Thomas; Stark, Birgit; Cullheim, Staffan; Hammarberg, Henrik
- Abstract
During nerve growth, axons are dependent upon contact with matrix components, such as laminins, for elongation, guidance, and trophic support. Semiquantitative in situ hybridization histochemistry and immunohistochemistry (IHC) were used to identify laminin chains in normal peripheral nerves, during postnatal development, after sciatic nerve transection (SNT), and after sciatic nerve crush (SNC). Laminin α2, α4, β1, β2, and γ1 chain mRNAs were all expressed at high levels in newborn rat sciatic nerves with declining levels during later developmental stages. At the adult stage, no laminin chain mRNA was detectable. Of interest, the mRNA levels for α4 chain declined faster than those for α2. After SNT, laminin α2, α4, β1, and γ1 mRNA levels were up-regulated at the site of the injury, with the most profound reaction in the proximal nerve stump. Laminin α2 and α4 chains differed in that the mRNA levels of α4 were up-regulated earlier and declined quicker, whereas α2 had a later onset, with high levels remaining even after 6 weeks. After SNC, there was an initial up-regulation of the same laminin chain mRNAs as after SNT in the nerve, however, less intense, and at 6 weeks after SNC, all laminin mRNA levels studied had returned to normal. IHC of adult human normal and transected peripheral nerves stained positive for laminin α2, α4, β1, and γ1 chains in close relation to neurofilament labeled axons. Laminin α3, α4, α5, β1, β2, and γ1 chains were found in blood vessel-like structures and α3, α4, α5, β2, and γ1 in the perineurium. These results and a previously published description of integrin regulation in spinal motoneurons suggest that both laminin-2 (α2β1γ1) and laminin-8 (α4β1γ1) are important for the postnatal nerve development and axonal regeneration after injury and that laminin-8 may have important functions especially early postnatally and early after adult nerve lesion. J. Comp. Neurol. 454:284-293, 2002. © 2002 Wiley-Liss, Inc.
- Publication
Journal of Comparative Neurology, 2002, Vol 454, Issue 3, p284
- ISSN
0021-9967
- Publication type
Article
- DOI
10.1002/cne.10434