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- Title
Starved human fibroblasts secrete acidic proteins inducing post re-feeding proliferation and in vitro cell migration: A potential tool for wound healing.
- Authors
Golpour, Monireh; Fattahi, Sadegh; Niaki, Haleh Akhavan; Hadipoor, Abbas; Abedian, Zeinab; Ahangarian, Gholam Reza; Parsian, Hadi; Mosapour, Abbas; Khorasani, Hamid Reza; Vaziri, Hamid Reza; Bijani, Ali; Mostafazadeh, Amrollah
- Abstract
Background Information There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. Results After re-feeding, 16 and 72 h serum-starved fibroblasts showed the highest and lowest proliferation rates, 1.59 and 0.51-fold difference compared to the non-starved control, respectively ( P < 0.05). However, the latest value could be normalised by incubating cells with 16 h-starved fibroblast cell culture supernatant (16-SFS), prior to re-feeding. A strong correlation was found between total protein level in starved fibroblast culture supernatants and post re-feeding proliferation rates ( r2 = 0.90, P < 0.001). Two-dimensional gel electrophoresis analysis of 16-SFS confirmed the presence of proteins with relative molecular weights of 10-120 kDa and p I ranging from 4 to 6. A significant difference in calcium influx course was found between 16-SFS and the negative control (Dulbecco's Modified Eagle Medium) ( P < 0.05). There was no significant difference in Ca2+ concentrations after 1 h between non-starved controls and 16-SFS-treated fibroblasts. The scratch test demonstrated that the 16-SFS is able to induce fibroblast migration. Conclusions We concluded that human starved fibroblasts secrete proteins that are able to induce post re-feeding cell proliferation and fibroblasts migration, probably through the induction of a sustained calcium influx. This is worth being considered as a potential tool for wound healing.
- Subjects
WOUND healing; CELL migration; CELL proliferation; THERAPEUTIC use of proteins; FIBROBLASTS; CELL culture; GEL electrophoresis
- Publication
Biology of the Cell (Wiley-Blackwell), 2014, Vol 106, Issue 5, p139
- ISSN
0248-4900
- Publication type
Article
- DOI
10.1111/boc.201300063