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- Title
Lymphocyte response to pokeweed mitogen in chronic lymphocytic leukemia.
- Authors
Lamberson, Harold V.; Davey, Frederick R.; Schreck, Celeste M.; Zamkoff, Kenneth; Kurec, Anthony S.; Lamberson, H V; Davey, F R; Schreck, C M; Zamkoff, K; Kurec, A S
- Abstract
The response of lymphocyte subpopulations to pokeweed mitogen (PWM) was studied in normal volunteers and patients with B-cell chronic lymphocytic leukemia (CLL). Since unfractionated peripheral blood mononuclear (PBM) cells from CLL patients consist of a markedly increased proportion of B-lymphocytes and a decreased proportion of T-lymphocytes, enriched fractions of CLL B-cells and CLL T-cells were cultured in 1:1 proportions in autologous and allogeneic combinations with normal B-cell and T-cell-enriched fractions. Cultures containing normal B-cells with either autologous or allogeneic normal T-cells responded well to PWM. CLL T-cells were capable of providing a helper function for both proliferation and differentiation of normal B-cells, which was not significantly different from that provided by allogeneic normal T-cells. CLL B-lymphocytes were unresponsive to PWM when cultured in the presence of either autologous CLL T-lymphocytes or allogeneic normal T-lymphocytes. The responsiveness of CLL B-cells was not restored by the addition of normal peripheral blood monocytes to the cultures. These experiments indicate that there is an intrinsic B-cell defect which prevents CLL B-lymphocytes from responding to PWM.
- Publication
Cancer (0008543X), 1984, Vol 53, Issue 11, p2481
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(19840601)53:11<2481::AID-CNCR2820531120>3.0.CO;2-Z