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- Title
Presence of Anti-La (SS-B) Is Associated with Binding to the 13-kD Carboxyl Terminus of 60-kD Ro(SS-A) in Systemic Lupus Erythematosus.
- Authors
Dickey, W. Darryl; van Egmond, Juliet E.; Hardgrave, Kathy L.; Harley, John B.; Scofield, R. Hal
- Abstract
In systemic lupus erythematosus (SLE) clinical manifestations, autoantibody production, and immunogenetics are inter-related. The ability to study parts of the autoimmune response may allow a more detailed understanding of these relationships. We undertook this study to determine whether the fine specificity of the autoimmune response to 60-kD Ro(SS-A) was related to the presence of other autoantibodies. We screened 74 patients with SLE for antibodies to the carboxyl 13-kD terminal of 60-kD Ro(SS-A) (13 kD). Twenty-five sera had such antibodies. This reactivity was distinguished by the presence of not only anti-Ro(SS-A) but also other antibodies. All nine sera with Ro(SS-A) and La(SS-B) Ouchterloney immunodiffusion precipitins bound 13-kD (p = 0.01), whereas 10 of 11 sera with both anti-Ro(SS-A) and anti-La(SS-B) as determined by immunosorbent assay bound 13-kD (p = 0.002). Inhibition studies demonstrated that antibodies binding the 13-kD fragment bound the 60-kD Ro(SS-A) protein but did not bind the La(SS-B) protein. Thus, anti-La(SS-B) was found in those sera that bound epitopes within the 13-kD carboxyl terminal of 60-kD Ro(SS-A). These data suggest a structural basis by which anti-Ro(SS-A) and anti-La(SS-B) are coupled in SLE.
- Subjects
SYSTEMIC lupus erythematosus; IMMUNOGENETICS; AUTOANTIBODIES; EPITOPES; IMMUNODIFFUSION; IMMUNE response
- Publication
Journal of Investigative Dermatology, 1993, Vol 100, Issue 4, p412
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12472055