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- Title
Bacteriostatic Activity of BCG/PPD-Activated Macrophages Against Mycobacterium fortuitum Does Not Involve Reactive Nitrogen or Oxygen Intermediates.
- Authors
Nibbering, P. H.; Yoshida, S.-I.; Van Den Barselaar, M. T.; Van Furth, R.
- Abstract
Mycobacteria preferentially reside in resident macrophages whereas activated macrophages are presumed to eliminate the bacteria effectively. The aim of the present study was to determine the antibacterial activities of resident and activated murine peritoneal macrophages against <em>Mycobacterium fortuitum</em> and the intracellular mechanisms involved. After phagocytosis <em>M. fortuitum</em> could not be killed by either BCG/PPD-activated and IFN-γ-activated macrophages and resident macrophages. The mycobacteria did not multiply in BCG/PPD-activated macrophages and the rate of proliferation of <em>M. fortuitum</em> in IFN-γ-activated macrophages was only slightly inhibited compared to that in resident macrophages. Experiments with selective inhibitors of the production of reactive nitrogen intermediates (RNI) and reactive oxygen intermediates (ROI) demonstrated that these factors are not essential for the mycobacteriostatic activity of BCG/PPD-activated macrophages. After phagocytosis of <em>M. fortuitum</em>, BCG/PPD-activated and IFN-γ-activated macrophages produced substantial amounts of both RNI and ROI. No correlation was found between the levels of these intermediates and the proliferation of <em>M. fortuitum</em> in the macrophages. In conclusion, BCG/PPD-activated macrophages are bacteriostatic, but not bacteriocidal for <em>M. fortuitum</em> and the former does not involve reactive nitrogen and oxygen intermediates.
- Subjects
MACROPHAGES; MYCOBACTERIUM; PHAGOCYTOSIS; MYCOBACTERIA; ANTIGEN presenting cells
- Publication
Scandinavian Journal of Immunology, 1994, Vol 40, Issue 2, p187
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1994.tb03449.x