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- Title
Binding of a Bacterial Acylpoly(l,3)Galactoside to Human Blood Leucocytes.
- Authors
Hmama, Z.; Kouassi, E.; Panaye, G.; Delassan, S.; Normier, G.; Binz, H.; Revillard, J.-P.
- Abstract
The binding of a 34-kDa (mol. wt.) acylpoly( 1,3)galactoside (APG)extracted from a membrane proteoglycan of Klebsiella pneumoniae to human blood leucocytes was investigated. APG is made of a long poly(1.3)galactose chain, a core-like region and a lipid moiety which comprises two glucosamine residues bound to a phosphate group and two βOH myristic acids. Fluoresceinated PG was shown to bind preferentially to monocytes and to a lesser extent to polymorphonuclear neutrophils, as determined by flow cytometry. Binding of fluoresceinated APG was inhibited by unlabelled APG: it was concentration dependent, but not saturable, with rapid kinetics. It occurred at +4°C but was markedly increased at 37°C. It involved trypsin-sensitive molecules on the membrane of monocytes. Neither the parent proteoglycan nor lipopolysaccharide from K. pneumoniae or Salmonella minnesota competed for APG binding. A minor non-specific binding to lymphocytes, occurring predominantly on B cells, was observed. Unlike that of lipopolysaccharide, the APG binding was not blocked by polymyxin B sulphate. Interaction between the galactose chain of APG and the galactose receptor does not account for the binding of APG to monocytes because the galactose receptor (Mac-2) is expressed at high density on activated macrophages but not on monocytes. Despite its strong binding to human blood monocytes, APG displayed a much weaker activity than K. pneumoniae membrane proteoglycan with respect to induction of monocyte cytokine synthesis. When administered as a Technetium 99 conjugate, APG was shown to label inflammatory loci in experimental animals, and its property as a marker of macrophages is currently being evaluated in clinical trials.
- Subjects
ENDOTOXINS; LEUCOCYTES; LEUCOCYTE motility; GALACTOSE; GLYCOLIPIDS; GLYCOCONJUGATES; LIPIDS
- Publication
Scandinavian Journal of Immunology, 1992, Vol 36, Issue 1, p11
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1992.tb02935.x