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- Title
IgG Rheumatoid Factor-Secreting Lymphocytes in Rheumatoid Arthritis: Evaluation of a Haemolytic Plaque-Forming Cell Technique.
- Authors
Petersen, J.; Heilmann, C.; Bjerrum, O. J.; Ingemann-Hansen, T.; Halkjær-Kristensen
- Abstract
A haemolytic plaque-forming cell (PFC) assay detecting human B lymphocytes secreting IgG rheumatoid factor (RF) was established using sheep erythrocytes (SRBC) sensitized with rabbit IgG, developing rabbit anti-human IgG, and complement. IgG-RF PFC were only demonstrated with IgG-depleted guinea-pig serum as the source of complement. Cells spontaneously secreting IgG-RF were found among synovial fluid mononuclear cells (mean, 134/106) and synovial tissue mononuclear cells (mean, 1775/106) from patients with rheumatoid arthritis, whereas few were recorded among blood lymphocytes (mean, 3/106). The experiments revealed that the RF-IgG PFC were protein-synthesizing B lymphocytes. The antibody specificity of the secreted IgF-RF was verified by the inhibitory effect of exogenous human and rabbit IgG on PFC formation.
- Subjects
RHEUMATOID factor; ANTI-immunoglobulin autoantibodies; LYMPHOCYTES; ERYTHROCYTES; RHEUMATOID arthritis; JOINT diseases
- Publication
Scandinavian Journal of Immunology, 1983, Vol 17, Issue 5, p471
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1983.tb00814.x