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- Title
A Synergistic Polyclonal Response to Dextran Sulphate and Lipopolysaccharide.
- Authors
Bergstedt-Lindqvist, S.; Fernandez, C.; Severinson, E.
- Abstract
The effects of the simultaneous addition of lipopolysaccharide (LPS) and dextran sulphate (DxS) were studied in a low-cell density culture system, which enables extensive cell proliferation and high immunoglobulin secretion. Using these two mitogens, a synergistic response was observed, with regard to both cell division and IgM secretion. However, only a very low IgG production could be detected. This was caused by the extensive cell proliferation, leading to suboptimal culture conditions. Thus, when the blasts were recultured at a lower cell density or when a lower initial cell concentration was used, a high IgG response was obtained. The synergistic response induced by LPS plus DxS was independent of T cells. Furthermore, no apparent need for phagocytic cells was found. Both the LPS- and the LPS plus DxS-induced activation led to a switch, preferentially to IgG2b and IgG3 secretion. This subclass pattern was not changed when the cultures were lacking functional T cells.
- Subjects
POLYSACCHARIDES; BIOPOLYMERS; CELL proliferation; CELL division; MITOGENS; MITOSIS
- Publication
Scandinavian Journal of Immunology, 1982, Vol 15, Issue 5, p439
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1982.tb00669.x