We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Ovalbumin Immunity in the Rat: Simultaneous Testing of IgM, IgG and IgE Response Measured by ELISA and Delayed-type Hypersensitivity.
- Authors
Vos, J. G.; Boerkamp, J.; Buys, J.; Steerenberg, P. A.
- Abstract
After immunization of Wistar rats with different concentrations of ovalbumin (OA) emulsified in complete H37Ra adjuvant, the optimal antigen dose for specific IgM, IgG and IgE production and for delayed-type hypersensitivity appeared to be the same. It is therefore concluded that in the rat these function tests can be combined, which decreases the number of experimental animals needed. For the determination of IgM, IgG and IgE antibodies to OA, the enzyme-linked-immunosorbent assay (ELISA) was used, which proved to be a specific method. In addition, using a multi-channel photometer, it is shown that specific antibody concentrations can be readily quantified by micro-ELISA instead of using end-point titrations.
- Subjects
IMMUNOGLOBULIN G; ENZYME-linked immunosorbent assay; IMMUNOLOGICAL adjuvants; PATHOLOGICAL physiology; LABORATORY animals; VOLUMETRIC analysis
- Publication
Scandinavian Journal of Immunology, 1980, Vol 12, Issue 4, p289
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00069.x