We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Airway disease decreases the therapeutic potential of epithelial stem cells.
- Authors
Zhang, Lisa; Kelly, Natalie; Shontz, Kimberly M; Hill, Cynthia L.; Stack, Jacob T.; Calyeca, Jazmin; Matrka, Laura; Miller, Audrey; Reynolds, Susan D; Chiang, Tendy
- Abstract
Backgorund: Tissue-engineered tracheal grafts (TETG) can be recellularized by the host or pre-seeded with host-derived cells. However, the impact of airway disease on the recellularization process is unknown. Methods: In this study, we determined if airway disease alters the regenerative potential of the human tracheobronchial epithelium (hTBE) obtained by brushing the tracheal mucosa during clinically-indicated bronchoscopy from 48 pediatric and six adult patients. Results: Our findings revealed that basal cell recovery and frequency did not vary by age or region. At passage 1, all samples produced enough cells to cellularize a 3.5 by 0.5 cm2 graft scaffold at low cell density (~ 7000 cells/cm2), and 43.75% could cellularize a scaffold at high cell density (~ 100,000 cells/cm2). At passage 2, all samples produced the number of cells required for both recellularization models. Further evaluation revealed that six pediatric samples (11%) and three (50%) adult samples contained basal cells with a squamous basal phenotype. These cells did not form a polarized epithelium or produce differentiated secretory or ciliated cells. In the pediatric population, the squamous basal cell phenotype was associated with degree of prematurity (< 28 weeks, 64% vs. 13%, p = 0.02), significant pulmonary history (83% vs. 34%, p = 0.02), specifically with bronchopulmonary dysplasia (67% vs. 19%, p = 0.01), and patients who underwent previous tracheostomy (67% vs. 23%, p = 0.03). Conclusions: In summary, screening high-risk pediatric or adult population based on clinical risk factors and laboratory findings could define appropriate candidates for airway reconstruction with tracheal scaffolds. Level of evidence. Level III Cohort study.
- Subjects
EPITHELIAL cells; STEM cells; CHILD patients; BRONCHOPULMONARY dysplasia; AIRWAY (Anatomy)
- Publication
Respiratory Research, 2024, Vol 25, Issue 1, p1
- ISSN
1465-9921
- Publication type
Article
- DOI
10.1186/s12931-024-02667-8