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- Title
Morphology and viability of pollen grains from wild varieties of pineapple.
- Authors
Soares, Taliane Leila; de Souza, Everton Hilo; Rossi, Monica Lanzoni; Duarte Souza, Fernanda Vidigal
- Abstract
The aim of this research was to evaluate the pollen grains viability from accessions of wild pineapple using in vitro pollen germination and the growth of the polinic tube in two different temperatures and acetic carmine, in addition, the study aimed to recognize the pattern of pollen morphology of some pineapple varieties. Samples from six pineapple accessions.' Silvestre-25, 1-26/803 (Ananas macrodonles), igor (A. comosus var. erectifolius), Ananás Santo Amaro, FRF-22 and FRF-32 (A. comosus var bracteatus) were used. The pollen grains were inoculated onto medium culture containing 0.01% of sucrose, 0.01% HyBOr, 0.01% KNO3, 0.03% Ca(NO3)2.4H20, solidified with 1% agar and pH adjusted to 6.5. Two incubation temperatures, 25°C e 30°C were evaluated. Pollen viability was evaluated by staining with 2% acetic carmine. All Varieties of A. comosus showed pollen grains of medium size, radial symmetry, oval to spheroidal, a,nbit 2-porate, subprolate, exine eutectic, reticulate, heterobrochate, lumens ranging front polygonal to rounded, grain-free, except for two accessions of A. macrodontes that showed big size. The best germination rates were obtained for A. macrodontes when compared to erectifolius and bracteatus. The majority of accessions presented pollen viability higher than 76% using the acetic carmine technique.
- Subjects
PINEAPPLE; POLLEN morphology; GERMINATION; POLLEN tube; PLANT inoculation
- Publication
Ciência Rural, 2011, Vol 41, Issue 10, p1744
- ISSN
0103-8478
- Publication type
Article