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- Title
A sequence element that tunes Escherichia coli tRNA<sup>Ala</sup><sub>GGC</sub> to ensure accurate decoding.
- Authors
Ledoux, Sarah; Olejniczak, Mikołaj; Uhlenbeck, Olke C
- Abstract
Mutating the rare A32-U38 nucleotide pair at the top of the anticodon loop of Escherichia coli tRNAAlaGGC to a more common U32-A38 pair results in a tRNA that performs almost normally on cognate codons but is unusually efficient in reading near-cognate codons. Pre–steady state kinetic measurements on E. coli ribosomes show that, unlike the wild-type tRNAAlaGGC, the misreading mutant tRNAAlaGGC shows rapid GTP hydrolysis and no detectable proofreading on near-cognate codons. Similarly, tRNAAlaGGC mutated to contain C32-G38, a pair that is found in some bacterial tRNAAlaGGC sequences, was able to decode only the cognate codons, whereas tRNAAlaGGC containing a more common C32-A38 pair was able to decode all cognate and near-cognate codons tested. We propose that many of the phylogenetically conserved sequence elements present in each tRNA have evolved to suppress translation of near-cognate codons.
- Subjects
ESCHERICHIA coli; NUCLEOTIDES; TRANSFER RNA; NUCLEOTIDE sequence; GENETIC mutation
- Publication
Nature Structural & Molecular Biology, 2009, Vol 16, Issue 4, p359
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.1581