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- Title
Survival and post-warming in vitro competence of human oocytes after high security closed system vitrification.
- Authors
Munck, N.; Verheyen, G.; Landuyt, L.; Stoop, D.; Velde, H.
- Abstract
Purpose: To compare two vitrification methods and two warming methods for human oocyte vitrification using a high security closed device in terms of survival, fertilization and embryo development. Methods: For vitrification, oocytes were (1) immediately placed in equilibration solution or (2) they were gradually exposed to the cryoprotectants. For warming, oocytes were placed (1) in a 25 μl preheated (37 °C) thawing solution droplet that was put at room temperature for 1 min once the oocytes were inside or (2) in a 150 μl droplet for 1 minute at 37 °C. Results: Survival and preimplantation development were significantly lower when warming was performed in a small preheated droplet. There was no significant difference in survival and embryo development between the gradual or direct exposure to cryoprotectants. Conclusions: Using this high security closed vitrification device a 90 % survival rate can be achieved when the oocytes are immediately warmed in a large volume at 37 °C.
- Subjects
OVUM; HUMAN in vitro fertilization; GENETIC engineering; CRYOPROTECTIVE agents; PREIMPLANTATION genetic diagnosis; EMBRYOLOGY
- Publication
Journal of Assisted Reproduction & Genetics, 2013, Vol 30, Issue 3, p361
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-013-9930-3