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- Title
β-1,4-Galactosyltransferase I involved in Schwann cells proliferation and apoptosis induced by tumor necrosis factor-alpha via the activation of MAP kinases signal pathways.
- Authors
Yuan, Qin; Yang, Huiguang; Cheng, Chun; Li, Chunmiao; Wu, Xiujie; Huan, Weipeng; Sun, Huiqing; Zhou, Zhengming; Wang, Yingying; Zhao, Yunhong; Lu, Xiang; Shen, Aiguo
- Abstract
β-1,4-galactosyltransferase-I (β-1,4-GalT-I) plays a critical role in the initiation and maintenance of peripheral nervous system inflammatory reaction. However, the exact function of β-1,4-GalT-I in the regulation of SCs proliferation and apoptosis remains unclear. In this study, we found that low concentration of tumor necrosis factor-alpha (TNF-α) induced SCs proliferation, while high concentration of TNF-α induced SCs apoptosis. Meanwhile, the expressions of β-1,4-GalT-I, TNFR1, and TNFR2 were changed following. When β-1,4-GalT I overexpression, low concentration of TNF-α-induced SCs proliferation was partially repressed. Concurrently, the activity of ERK1/2 was decreased. While knocking down β-1,4-GalT I expression, high concentration of TNF-α-induced SCs apoptosis was partially rescued. Consistent with this, the activity of P38 and JNK were decreased. We also found anti-TNFR2 antibody suppressed low concentration of TNF-α-induced SCs proliferation, while anti-TNFR1 antibody inhibited high concentration of TNF-α-induced SCs apoptosis. Thus, present data show that β-1,4-GalT I may play an important role in SCs proliferation and apoptosis induced by TNF-α via different signal pathways and TNFR.
- Subjects
GALACTOSYLTRANSFERASES; SCHWANN cells; TUMOR necrosis factors; CELL proliferation; APOPTOSIS
- Publication
Molecular & Cellular Biochemistry, 2012, Vol 365, Issue 1/2, p149
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-012-1254-6