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- Title
Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801.
- Authors
Goshima, Tetsuya; Akao, Takeshi; Ichikawa, Eri; Tamura, Hiroyasu; Nakamura, Ryo; Hirata, Dai; Kume, Kazunori; Mizunuma, Masaki; Ohya, Yoshikazu; Okada, Hiroki; Hasuda, Hirokazu; Inahashi, Masaaki; Okazaki, Naoto; Shimoi, Hitoshi
- Abstract
In high-quality sake brewing, the cerulenin-resistant sake yeast K1801 with high ethyl caproate-producing ability has been used widely; however, K1801 has a defective spindle assembly checkpoint (SAC). To identify the mutation causing this defect, we first searched for sake yeasts with a SAC-defect like K1801 and found that K13 had such a defect. Then, we searched for a common SNP in only K1801 and K13 by examining 15 checkpoint-related genes in 23 sake yeasts, and found 1 mutation, R48P of Cdc55, the PP2A regulatory B subunit that is important for the SAC. Furthermore, we confirmed that the Cdc55-R48P mutation was responsible for the SAC-defect in K1801 by molecular genetic analyses. Morphological analysis indicated that this mutation caused a high cell morphological variation. But this mutation did not affect the excellent brewing properties of K1801. Thus, this mutation is a target for breeding of a new risk-free K1801 with normal checkpoint integrity. K1801 (triploid) having 2 copies of R48P-typecdc55and 1 copy of R48-typecdc55showed a defective spindle assembly checkpoint (SAC). Cdc55-R48P is semidominant mutation in the SAC function.
- Subjects
BREWING; CAPROATES; SPINDLE apparatus
- Publication
Bioscience, Biotechnology & Biochemistry, 2016, Vol 80, Issue 8, p1657
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2016.1184963