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- Title
Surfactant protein C metabolism in human infants and adult patients by stable isotope tracer and mass spectrometry.
- Authors
Simonato, Manuela; Baritussio, Aldo; Pioselli, Barbara; Ori, Carlo; Catinella, Silvia; Carnielli, Virgilio; Cogo, Paola
- Abstract
Surfactant protein C (SP-C) is deemed as the surfactant protein most specifically expressed in type II alveolar epithelial cells and plays an important role in surfactant function. SP-C turnover in humans and its meaning in the clinical context have never been approached. In this study, we used mass spectrometry to investigate SP-C turnover in humans. We studied four infants and eight adults requiring mechanical ventilation. All patients had no lung disease. Patients received a 24-h continuous infusion of C-leucine as precursor of SP-C, and serial tracheal aspirates and plasma samples were obtained every 6 h till 48 h. SP-C was isolated from tracheal aspirates by sorbent-phase chromatography. C-leucine SP-C enrichment could be successfully measured in three infant and in four adult samples by using mass spectrometry coupled with a gas chromatographer. Median SP-C fractional synthesis rate, secretion time, and peak time were 15.7 (14.1-27.5) %/day, 6.0 (4.7-11.5) h, and 24 (20-27) h. In conclusion, this study shows that it is feasible to accurately determine SP-C turnover in humans by stable isotopes.
- Subjects
PULMONARY surfactant-associated protein C; EPITHELIAL cells; MASS spectrometry; AMINO acids; SURFACE active agents
- Publication
Analytical & Bioanalytical Chemistry, 2014, Vol 406, Issue 25, p6225
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-014-8014-2