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- Title
Blastocyst complementation reveals that NKX2‐1 establishes the proximal‐peripheral boundary of the airway epithelium.
- Authors
Li, Enhong; Ustiyan, Vladimir; Wen, Bingqiang; Kalin, Gregory T.; Whitsett, Jeffrey A.; Kalin, Tanya V.; Kalinichenko, Vladimir V.
- Abstract
Background: Distinct boundaries between the proximal conducting airways and more peripheral‐bronchial regions of the lung are established early in foregut embryogenesis, demarcated in part by the distribution of SOX family and NKX2‐1 transcription factors along the cephalo‐caudal axis of the lung. We used blastocyst complementation to identify the role of NKX2‐1 in the formation of the proximal‐peripheral boundary of the airways in mouse chimeric embryos. Results: While Nkx2‐1−/− mouse embryos form primordial tracheal cysts, peripheral pulmonary structures are entirely lacking in Nkx2‐1−/−mice. Complementation of Nkx2‐1−/− embryos with NKX2‐1‐sufficient embryonic stem cells (ESCs) enabled the formation of all tissue components of the peripheral lung but did not enhance ESC colonization of the most proximal regions of the airways. In chimeric mice, a precise boundary was formed between NKX2‐1‐deficient basal cells co‐expressing SOX2 and SOX9 in large airways and ESC‐derived NKX2‐1+SOX9+ epithelial cells of smaller airways. NKX2‐1‐sufficient ESCs were able to selectively complement peripheral, rather than most proximal regions of the airways. ESC complementation did not prevent ectopic expression of SOX9 but restored β‐catenin signaling in Nkx2‐1−/− basal cells of large airways. Conclusions: NKX2‐1 and β‐catenin function in an epithelial cell‐autonomous manner to establish the proximal‐peripheral boundary along developing airways. Key Findings: Pluripotent ESCs generate peripheral lung tissue in Nkx2‐1‐/‐ chimeras.NKX2‐1 establishes the proximal‐peripheral boundary of the airways.ESCs complementation restores b‐catenin signaling and formation of the peripheral acinar buds in Nkx2‐1‐/‐ embryos.NKX2‐1 restricts SOX9 in basal cells to enhance patterning of the peripheral vs proximal lung.
- Subjects
EMBRYONIC stem cells; BLASTOCYST; EPITHELIUM; CELL aggregation; EPITHELIAL cells
- Publication
Developmental Dynamics, 2021, Vol 250, Issue 7, p1001
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.298