We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Downregulation of MicroRNA-19b Contributes to Angiotensin II-Induced Overexpression of Connective Tissue Growth Factor in Cardiomyocytes.
- Authors
Gao, Song; Liu, Tie-wen; Wang, Zhe; Jiao, Zhen-yu; Cai, Jun; Chi, Hong-jie; Yang, Xin-chun
- Abstract
Objectives: The present study was designed to decipher the molecular mechanisms underlying angiotensin (Ang) II-induced overexpression of connective tissue growth factor (CTGF) in cultured cardiomyocytes. Methods: Cardiomyocytes isolated from 1- to 3-day-old neonatal rats were cultured and treated with 100 nM Ang II with or without pretreatment with 10 nM telmisartan, an Ang II type 1 receptor antagonist. The role of microRNA (miR)-19b in the regulation of Ang II-induced CTGF expression was evaluated in cultured cardiomyocytes with quantitative real-time reverse transcription polymerase chain reaction and Western blot analysis. Results: We provide several lines of evidence to show that miR-19b contributes to the Ang II-induced overexpression of CTGF in cultured cardiomyocytes. Firstly, administration of Ang II decreased the level of miR-19b dramatically (p < 0.05 vs. control), which was abolished by telmisartan. Secondly, Ang II increased the level of CTGF significantly (p < 0.05 vs. control), which was also prevented by pretreatment with telmisartan. Thirdly, overexpression of miR-19b decreased CTGF levels (p < 0.05 vs. control). Finally, transfection of miR-19b into cardiomyocytes prevented the upregulation of CTGF induced by Ang II. Conclusion: Downregulation of miR-19b contributes to Ang II-induced overexpression of CTGF in cultured cardiomyocytes. © 2013 S. Karger AG, Basel
- Subjects
ANGIOTENSINS; ANGIOTENSIN II; MICRORNA; CONNECTIVE tissue growth factor; HEART cells; POLYMERASE chain reaction; WESTERN immunoblotting
- Publication
Cardiology, 2014, Vol 127, Issue 2, p114
- ISSN
0008-6312
- Publication type
Article
- DOI
10.1159/000355429