We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differential expression of immunomodulatory galectin-1 in peripheral leukocytes and adult tissues and its cytosolic organization in striated muscle.
- Authors
Dias-Baruffi, Marcelo; Stowell, Sean R.; Shuh-Chyung Song; Arthur, Connie M.; Moonjae Cho; Rodrigues, Lilian C.; Montes, Marlise A. B.; Rossi, Marcos A.; James, Judith A.; McEver, Rodger P.; Cummings, Richard D.
- Abstract
Galectin-1 (Gal-1) is important in immune function and muscle regeneration, but its expression and localization in adult tissues and primary leukocytes remain unclear. To address this, we generated a specific monoclonal antibody against Gal-1, termed αhGal-1, and defined a sequential peptide epitope that it recognizes, which is preserved in human and porcine Gal-1, but not in murine Gal-1. Using αhGal-1, we found that Gal-1 is expressed in a wide range of porcine tissues, including striated muscle, liver, lung, brain, kidney, spleen, and intestine. In most types of cells, Gal-1 exhibits diffuse cytosolic expression, but in cells within the splenic red pulp, Gal-1 showed both cytosolic and nuclear localization. Gal-1 was also expressed in arterial walls and exhibited prominent cytosolic and nuclear staining in cultured human endothelial cells. However, human peripheral leukocytes and promyelocytic HL60 cells lack detectable Gal-1 and also showed very low levels of Gal-1 mRNA. In striking contrast, Gal-1 exhibited an organized cytosolic staining pattern within striated muscle tissue of cardiac and skeletal muscle and colocalized with sarcomeric actin on I bands. These results provide insights into previously defined roles for Gal-1 in inflammation, immune regulation and muscle biology.
- Subjects
LEUCOCYTES; MONOCLONAL antibodies; PEPTIDES; STRIATED muscle; MUSCULOSKELETAL system
- Publication
Glycobiology, 2010, Vol 20, Issue 5, p507
- ISSN
0959-6658
- Publication type
Article
- DOI
10.1093/glycob/cwp203