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- Title
STUDIES ON CLINICAL AND MOLECULAR DIAGNOSIS IN NATURALLY OCCURING CASES OF CANINE MONOCYTIC EHRLICHIOSIS.
- Authors
Kumari, Priyanka; Banerjee, P. S.; Garg, Rajat
- Abstract
Canine ehrlichiosis, caused by the rickettsial organisms, is one of the most prevalent vector borne infectious diseases of dogs around the globe. Ehrlichia canis is a Gram-negative coccoid to ellipsoidal bacterium, occurring intracytoplasmically, either singly or in compact inclusions (morulae) in monocytes and is mainly transmitted by the brown dog-tick Rhipicephalus sanguineus. The acute disease is clinically dianosed by clinical signs viz. fever, depression, lethargy, anorexia, lymphadenomegaly, splenomegaly and hemorrhagic tendencies. The latter is usually exhibited by dermal petechiae and ecchymoses and epistaxis. Ophthalmological lesions are frequent and include anterior uveitis, chorioretinitis, papilledem and retinal hemorrhage. Early diagnosis of E. canis infection during the acute phase ensures the best prognosis and usually leads to complete recovery. Though, microscopy is most widely used diagnostic tool under field conditions daily. The diagnosis of CME can be made by detecting typical intracytoplasmic morula in monocytes in blood smears by light microscopy. Unfortunately, the search for morula is difficult and time consuming and has been estimated to be successful in only about 4% of positive cases (Woody and Hoskins, 1991). Sensitive and specific molecular techniques were evaluated for the detection of antigens. Molecular diagnosis is done by specific published primer set, EC12-F and EC9-R was used in the primary PCR to amplify the sequence of 1500 bp fragment of 16S rRNA region (Wen et al, 1997). E. canis specific nested PCR was performed to amplify 389 bp fragment from primary PCR product using primer set ECA-F and HE3-R (Kawahara et al, 1999).
- Subjects
MOLECULAR diagnosis; EHRLICHIOSIS; VECTOR-borne diseases; SYMPTOMS; BROWN dog tick; TICK infestations
- Publication
Journal of Experimental Zoology India, 2024, Vol 27, Issue 1, p307
- ISSN
0972-0030
- Publication type
Article
- DOI
10.51470/jez.2024.27.1.307