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- Title
Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity.
- Authors
Farias, Eduardo F.; Ong, David E.; Ghyselinck, Norbert B.; Nakajo, Shigeo; Kuppumbatti, Yivarani S.; y Lopez, Rafael Mira; Kuppumbatti, Yuvarani S; Mira y Lopez, Rafael
- Abstract
<bold>Background: </bold>Retinoic acid receptor (RAR) activation induces cell differentiation and may antagonize cancer progression. Cellular retinol-binding protein I (CRBP-I) functions in retinol storage and its expression is lower in human cancers than in normal cells. We hypothesized that retinol storage might be linked to RAR activation and thus that lowered CRBP-I function might impair RAR activity and cell differentiation.<bold>Methods: </bold>Sarcoma virus 40-immortalized human mammary epithelial cells (MTSV1-7) devoid of CRBP-I were transfected with wild-type CRBP-I or CRBP-I point mutants with low RA binding affinity. The subcellular localization of CRBP-I was investigated in these cells and in wild-type or CRBP-I null mouse mammary epithelial cells (MECs), using indirect immunofluorescence and sucrose gradient fractionation. RAR activity was assessed using reporter gene assays. Acinar differentiation and in vivo tumor growth were assessed in reconstituted basement membrane and athymic mice, respectively.<bold>Results: </bold>In cells expressing wild-type CRBP-I but not the CRBP-I mutants, CRBP-I was found mainly in lipid droplets, the retinol storage organelle, and this localization was associated with promotion of retinol storage by wild-type CRBP-I only. RAR activity was higher and acinar differentiation was observed in cells expressing wild-type but not mutant CRBP-I. RAR antagonist treatment blocked and chronic RA treatment mimicked, the CRBP-I induction of cell differentiation. Finally, CRBP-I suppressed tumorigenicity in athymic mice.<bold>Conclusions: </bold>Physiologic RAR activation is dependent on CRBP-I-mediated retinol storage, and CRBP-I downregulation chronically compromises RAR activity, leading to loss of cell differentiation and tumor progression.
- Subjects
BREAST cancer; VITAMIN A; CELL differentiation; CANCER invasiveness; CARCINOGENESIS; ONCOLOGY; ANIMAL experimentation; BIOCHEMISTRY; BREAST; BREAST tumors; CARRIER proteins; CELL receptors; COMPARATIVE studies; EPITHELIAL cells; FLUORESCENT antibody technique; GENES; GENETIC techniques; PHENOMENOLOGY; RESEARCH methodology; MEDICAL cooperation; MICE; GENETIC mutation; RESEARCH; SUCROSE; EVALUATION research; DISEASE progression
- Publication
JNCI: Journal of the National Cancer Institute, 2005, Vol 97, Issue 1, p21
- ISSN
0027-8874
- Publication type
journal article
- DOI
10.1093/jnci/dji004