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- Title
三种不同饮食干预建立非酒精性脂肪肝模型.
- Authors
张丹; 吕俊衍; 邓溢; 周波; 耿坐涛; 罗粟风; 王金香; 文玉; 应昀晏; 文代艳; 马岚青
- Abstract
Objective To establish a non-alcoholic fatty liver disease(NAFLD) research of model in mice by three different dietary interventions,and to provide a reference for the mechanism research of NAFLD. Methods Sixty healthy male C57BL/6 mice were randomly divided into normal control group (N), high-fat diet group (HFD), folic acid deficiency group (FD) and high-homocysteine group (HCY), 15 in each group, and they were modeled for 14 weeks. The general situation of mice in groups were observed,the indexes of insulin and fasting glucose and hepatic triglyceride were measured, and the liver pathology was performed. Results Compared with the N group, the HFD group had a significant weight gain (P < 0.0001) and the HCY group had a significant weight loss(P < 0.05) after the intervention diet for 14 weeks. The liver pathological results showed that fatty degeneration appeared in the HFD group,FD group and HCY group. Insulin resistance index was significantly increased in the HFD group (P < 0.01),Hepatic triglyceride level was significantly increased in each model group (P < 0.05) . Conclusion Three different dietary interventions can successfully induce NAFLD mouse model with simple and reproducible methods to simulate NAFLD with different etiologies,provide an ideal animal model for the study of the mechanism and drug therapy of NAFLD.
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2020, Vol 41, Issue 1, p11
- ISSN
2095-610X
- Publication type
Article