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- Title
Studies on Optimization of Extracting Condition for Glucosinolates in Chinese Kale (Brassica alboglabra Bailey).
- Authors
LA Gui-xiao; KONG Hai-min; FANG Ping; YANG Tie-gang
- Abstract
Taking the orthogonal test design, this paper studied the effect of different extraction factors ( extract temperature, methanol concentrations of extract solution, extract time, and ratios between sample to be tested and extract solution) on glucosinolates (GSs) extraction the total content of GSs, total aliphatic GSs, and total indolyl GSs from bolting stems of Chinese kale ( Brassica alboglabra Bailey ) . Sample/ solution ratio and extract temperature had significant influence on the total content of GSs, total aliphatic GSs, and total indolyl GSs (P<0.05). While, methanol concentration and extract time had no significant influence on Glucosinolate extraction yield ( P >0.05). Considering the extraction effect and cost, we suggested the optimal extract temperature, ratio of sample to extract solution, methanol concentrations of extract solution, and extract time are 75T1, 1:45(g : mL), 70% and 5 min, respectively. Under the optimal condition, the total content of GSs, total aliphatic GSs, and total indolyl GSs are 8.31 µmol/g DW, 7.58 µmol/g DW, and 0.73 µmol/g DW, respectively.
- Subjects
BRASSICA; KALE; GLUCOSINOLATES; METHANOL; COLE crops
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2014, Vol 16, Issue 3, p150
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2013.622