We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Restricted trandsuction by bacteriophage P22 in Salmonella typhimurium.
- Authors
Smith-Keary, P. F.
- Abstract
1. In the transduction pro-401 (×) + some of the transductants are surrounded by several hundred small wild-type satellite colonies; these transductants spontaneously release phage which transduces pro-401 to wild-type at high frequency (HFT phage).2. When the HFT phage is used to infect pro-401 at very low multiplicities of infection, most of the transductants are defective lysogens and segregate proline-requiring phage-sensitive derivatives; these transductants are apparently heterogenotes. At higher multiplicities of infection, or with lysogenic recipients, a higher proportion of satellited transductants is found.3. The HFT phage preparations transduce only the proline region of the donor genome.4. The existence is inferred of a defective P22 particle specifically incorporating the proline region of the Salmonella chromosome; these defective particles can establish themselves as prophage and confer immunity upon the infected cell, but are unable to replicate unless a normal prophage is also present. Satellited transductants are lysogenic both for a normal and defective (proline region carrying) phage, and so on lysis release transducing phage.5. This system is compared with the λdg-gal and P1-dl-lac systems in E. coli.
- Publication
Genetics Research, 1966, Vol 8, Issue 1, p73
- ISSN
0016-6723
- Publication type
Article
- DOI
10.1017/S0016672300009927