We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Tremella polysaccharides inhibit cellular apoptosis and autophagy induced by Pseudomonas aeruginosa lipopolysaccharide in A549 cells through sirtuin 1 activation.
- Authors
Shi, Xiaolan; Wei, WENfENg; Wang, Ning
- Abstract
In the present study, the role of Tremella polysaccharides in cellular apoptosis and autophagy induced by Pseudomonas aeruginosa lipopolysaccharide (LPS) in human epithelial A549 lung‑cancer cells was investigated. Initially, it was demonstrated that LPS attenuated A549 cell viability in a time‑ and dose‑dependent manner. Furthermore, LPS induced apoptotic cell death and autophagy in A549 cells and increased reactive oxygen species (ROS) production in a time‑dependent manner. In addition, LPS treatment was demonstrated to markedly suppress sirtuin 1 (SIRT1) protein expression in A549 cells. Notably, it was demonstrated that Tremella polysaccharides activate SIRT1, leading to increased p62 expression, decreased p53 acetylation and B‑cell lymphoma 2‑associated X protein expression, and subsequently attenuate LPS‑induced apoptotic cell death and autophagy. The results of the present study demonstrated that Tremella polysaccharides activate SIRT1 and inhibit LPS‑induced ROS production, apoptosis and autophagy. This may have critical implications for the treatment of Pseudomonas aeruginosa infection.
- Subjects
LUNG cancer; TREMELLA; POLYSACCHARIDES; APOPTOSIS; PSEUDOMONAS aeruginosa; SIRTUINS
- Publication
Oncology Letters, 2018, Vol 15, Issue 6, p9609
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2018.8554