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- Title
Effect of BMAP-28 on human thyroid cancer TT cells is mediated by inducing apoptosis.
- Authors
DAQI ZHANG; LANLAN WAN; JINNAN ZHANG; CHANG LIU; HUI SUN
- Abstract
Thyroid cancer is the most common malignant endocrine tumor, with significant morbidity and mortality. Bovine myeloid antimicrobial peptide 28 (BMAP-28) is a cathelicidin that is found in bovine neutrophils. In the present study, the effect and relative mechanism of BMAP-28 on the human thyroid cancer TT cell line in vitro and in vivo were investigated. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry and a TT-xenograft mouse model were used in this study. The data obtained indicated that BMAP-28 significantly inhibited the proliferation of the TT cells in vitro. In addition, the Annexin V-fluorescein isothiocyanate/propidium iodide assay detected that BMAP-28 induced apoptotic effects in the TT cells. Moreover, the expression of activated caspase-3 and -9 was upregulated at the transcriptional and translational levels. Simultaneously, the expression of matrix metalloproteinase (MMP)3 and MMP9 was down-regulated following BMAP-28 treatment. Finally, BMAP-28 significantly prevented the tumor growth in the TT-xenograft mouse model. These results indicated that BMAP-28 could be a potential agent for the treatment of thyroid cancer.
- Subjects
THYROID cancer; APOPTOSIS; CASPASES; MATRIX metalloproteinases; ENDOCRINE gland cancer
- Publication
Oncology Letters, 2015, Vol 10, Issue 4, p2620
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2015.3612