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- Title
Isolation and Purification of Enzymatic Hydrolysates of Yam (Dioscorea opposita Thunb.) Proteins.
- Authors
Mengchen XU; Ke DING; Ying LU; Tao HAN
- Abstract
Using yam (Dioscorea opposita Thunb.) as the experimental material, enzymatic hydrolysates of yam proteins were prepared with alkaline protease, which were then isolated and purified by cellulose DE-52 anion exchange chromatography Sephadex G-50 gel chromatography. According to the results, four absorption peaks were obtained by cellulose DE-52 anion exchange chromatography, and fractions at each absorption peak were collected. Specifically, the reducing ability of four peaks demonstrated a descending order of peak 2 (P2) > peak 1 (P1) > peak 3 (P3) > peak 4 (P4). By Sephadex G-50 gel chromatography, P1 and P2 were isolated and purified with distilled water and Tris-HCl buffer, and two absorption peaks were obtained, respectively.
- Subjects
YAMS; PLANT protein analysis; HYDROLYSIS; PLANT enzyme analysis; AGRICULTURAL biotechnology
- Publication
Agricultural Biotechnology (2164-4993), 2016, Vol 5, Issue 1, p19
- ISSN
2164-4993
- Publication type
Article