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- Title
Evaluation of deoxyribonuclease 1-Like 3 as a potential regulator for immune activation in juvenile-onset systemic lupus erythematosus patients: a case-control study.
- Authors
Eissa, Eman; Medhat, Basma M.; Morcos, Botros; Dorgham, Dalia; Kandil, Rania; El-Ghobashy, Nehal; Kholoussi, Naglaa M.
- Abstract
Objectives: This study aimed to evaluate the expression pattern of Deoxyribonuclease1-Like 3 (DNase1L3), an endonuclease that catalyses the degradation of chromatin within apoptotic or necrotic cells, at the mRNA and protein levels and their association with the disease activity and inflammatory markers in Juvenile-onset systemic lupus erythematosus (jSLE) patients. Methods: 36 Juvenile-onset systemic lupus erythematosus patients and 25 healthy controls were included in the study. DNase1L3 and Interleukin (IL)-1β expression in peripheral blood were determined using qRT-PCR, while ELISA was used to determine plasma levels of DNase1L3 and tumour necrosis factor (TNF)-α. Results: DNase1L3 expression pattern at the mRNA and protein levels was significantly lower while IL-1β and TNF-a levels were significantly higher in Juvenile-onset systemic lupus erythematosus patients than healthy controls. There were significant negative associations between DNase1L3 expression at mRNA and protein levels with SLEDAI-2K of patients. Also, DNase1L3 plasma levels of patients were inversely correlated with IL-1β levels in a significant manner. Conclusion: DNase1L3 could be involved in the immune regulation of jSLE patients and could be considered as a potential regulator of immune activation in those patients.
- Subjects
EGYPT; BIOMARKERS; INTERLEUKINS; PROTEINS; CYTOKINES; ACADEMIC medical centers; DEOXYRIBONUCLEOSIDES; APOPTOSIS; CASE-control method; GENE expression; MESSENGER RNA; ENZYME-linked immunosorbent assay; TUMOR necrosis factors; DESCRIPTIVE statistics; SYSTEMIC lupus erythematosus; CHILDREN
- Publication
New Zealand Journal of Medical Laboratory Science, 2022, Vol 76, Issue 2, p78
- ISSN
1171-0195
- Publication type
Article