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- Title
Neutral ceramidase-enriched exosomes prevent palmitic acid-induced insulin resistance in H4 IIEC3 hepatocytes.
- Authors
Zhu, Qun; Zhu, Rongping; Jin, Junfei
- Abstract
Oversupply of free fatty acids such as palmitic acid ( PA) from the portal vein may cause liver insulin resistance. Production of reactive oxygen species plays a pivotal role in PA-induced insulin resistance in H4 IIEC3 hepatocytes. Recently, we found that exosomes secreted from INS-1 cells that were transfected with neutral ceramidase ( NCDase) plasmids had raised NCDase activity; these NCDase-enriched exosomes could inhibit PA-induced INS-1 cell apoptosis. Here, we showed that PA reduced insulin-stimulated tyrosine phosphorylation of insulin receptor substrate 2 and decreased insulin-stimulated uptake of the fluorescent glucose analog 2- NBDG, confirming that insulin resistance occurred in PA-treated H4 IIEC3 cells. Moreover, NCDase-enriched exosomes from INS-1 cells rescued PA-induced H4 IIEC3 insulin resistance and blocked PA-induced reactive oxygen species production in which ceramide was involved.
- Subjects
CERAMIDASES; EXOSOMES; PALMITIC acid; INSULIN resistance; LIVER cells
- Publication
FEBS Open Bio, 2016, Vol 6, Issue 11, p1078
- ISSN
2211-5463
- Publication type
Article
- DOI
10.1002/2211-5463.12125