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- Title
Formation of MgF<sub>3</sub><sup>−</sup>-dependent complexes between an AAA<sup>+</sup> ATPase and σ<sup>54</sup>
- Authors
Zhang, Nan; Buck, Martin
- Abstract
Abstract: The widely distributed bacterial σ54-dependent transcription regulates pathogenicity and numerous adaptive responses in diverse bacteria. Formation of the σ54-dependent open promoter complex is a multi-step process driven by AAA+ ATPases. Non-hydrolysable nucleotide analogues are particularly suitable for studying such complexity by capturing various intermediate states along the energy coupling pathway. Here we report a novel ATP analogue, ADP–MgF3−, which traps an AAA+ ATPase with its target σ54. The MgF3−-dependent complex is highly homogeneous and functional assays suggest it may represent an early transcription intermediate state valuable for structural studies.
- Subjects
GENETIC transcription; ADENOSINE triphosphatase; INTERMEDIATE state (Superconductors); NUCLEOTIDES; GENETIC regulation; PROMOTERS (Genetics)
- Publication
FEBS Open Bio, 2012, Vol 2, p89
- ISSN
2211-5463
- Publication type
Article
- DOI
10.1016/j.fob.2012.04.002