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- Title
A Cresyl Violet-Based Fluorescent Off-On Probe for the Detection and Imaging of Hypoxia and Nitroreductase in Living Organisms.
- Authors
Wan, Qiong ‐ Qiong; Gao, Xing ‐ Hui; He, Xin ‐ Yuan; Chen, Su ‐ Ming; Song, Yan ‐ Chao; Gong, Qiu ‐ Yu; Li, Xiao ‐ Hua; Ma, Hui ‐ Min
- Abstract
A new cresyl violet-based fluorescent off-on probe has been developed through a one-step synthesis for the detection of nitroreductase (NTR) and hypoxia. The detection mechanism is based on the NTR-catalyzed reduction of the probe to cresyl violet, accompanied with a large fluorescence enhancement at a long wavelength of 625 nm. The probe can detect NTR in aqueous solution with high selectivity and sensitivity, and the detection limit is 1 ng mL−1 NTR. Most importantly, the probe has been successfully used to image not only NTR and hypoxia in living cells, but also the distribution of NTR in zebrafish in vivo.
- Subjects
HYPOXEMIA; NITROREDUCTASES; CATALYSIS; FLUORESCENCE; ZEBRA danio; DIAGNOSIS
- Publication
Chemistry - An Asian Journal, 2014, Vol 9, Issue 8, p2058
- ISSN
1861-4728
- Publication type
Article
- DOI
10.1002/asia.201402364