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- Title
Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody.
- Authors
Ohshima, Shino; Mori, Syuuya; Shigenari, Atsuko; Miyamoto, Asuka; Takasu, Masaki; Imaeda, Noriaki; Nunomura, Satoshi; Okayama, Yoshimichi; Tanaka, Masafumi; Kitagawa, Hitoshi; Kulski, Jerzy K.; Inoko, Hidetoshi; Ando, Asako; Kametani, Yoshie
- Abstract
CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells with a likely role in cell survival, proliferation and differentiation. In order to study the differentiation activity of porcine CD117 hematopoietic cells in vitro and in vivo we prepared an anti-swine CD117 Mab (2A1) with high specificity for flow-cytometrical analysis. The 2A1 Mab did not recognize mouse or human mast cells suggesting that 2A1 is species-specific. Swine bone marrow (BM) CD117+ cells differentiated in vitro mainly into erythroid and monocyte lineages in the methylcellulose-based colony assay. When the swine BM CD117+ cells were transplanted in vivo into immunodeficient NOG (NOD/SCID/IL-2gcnull) mice, a significant amount of swine CD45+ leukocytes, including CD3 positive T cells, were developed in the mice. These results revealed that the swine BM CD117+ cells possess hematopoietic stem/progenitor activity and when monitored in immunodeficient mice or in vitro they can develop into lymphoid, erythroid, and myeloid cells efficiently with the new monoclonal antibody.
- Subjects
HEMATOPOIETIC stem cells; PROGENITOR cells; MONOCLONAL antibodies; C-kit protein; CYTOKINE receptors; CELL differentiation; CELL proliferation
- Publication
BioScience Trends, 2014, Vol 8, Issue 6, p308
- ISSN
1881-7815
- Publication type
Article
- DOI
10.5582/bst.2014.01084