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- Title
Evaluation of the Diagnostic Accuracy of a New Dengue IgA Capture Assay (Platelia Dengue IgA Capture, Bio-Rad) for Dengue Infection Detection.
- Authors
De Decker, Sophie; Vray, Muriel; Sistek, Viridiana; Labeau, Bhety; Enfissi, Antoine; Rousset, Dominique; Matheus, Séverine
- Abstract
Considering the short lifetime of IgA antibodies in serum and the key advantages of antibody detection ELISAs in terms of sensitivity and specificity, Bio-Rad has just developed a new ELISA test based on the detection of specific anti-dengue IgA. This study has been carried out to assess the performance of this Platelia Dengue IgA Capture assay for dengue infection detection. A total of 184 well-characterized samples provided by the French Guiana NRC sera collection (Laboratory of Virology, Institut Pasteur in French Guiana) were selected among samples collected between 2002 and 2013 from patients exhibiting a dengue-like syndrome. A first group included 134 sera from confirmed dengue-infected patients, and a second included 50 sera from non-dengue infected patients, all collected between day 3 and day 15 after the onset of fever. Dengue infection diagnoses were all confirmed using reference assays by direct virological identification using RT-PCR or virus culture on acute sera samples or on paired acute-phase sera samples of selected convalescent sera. This study revealed: i) a good overall sensitivity and specificity of the IgA index test, i.e., 93% and 88% respectively, indicating its good correlation to acute dengue diagnosis; and ii) a good concordance with the Panbio IgM capture ELISA. Because of the shorter persistence of dengue virus-specific IgA than IgM, these results underlined the relevance of this new test, which could significantly improve dengue diagnosis accuracy, especially in countries where dengue virus is (hyper-) endemic. It would allow for additional refinement of dengue diagnostic strategy. Author Summary: Dengue disease has become a major global public health concern, but an ideal diagnostic test that permits early and rapid diagnosis is not yet available. Improving diagnostic performance in this area is a major challenge and necessitates the development and evaluation of new efficient, accurate methods. According to the kinetics of dengue infection in serum, virus isolation and nucleic acid or antigen detection are the most specific diagnostic methods during the early acute stage of disease; serology is often used for diagnosis later in the course of infection. In order to provide an earlier and reliable dating of the dengue infection, few recent studies showed that the detection of specific IgA in the serum is a useful diagnostic parameter. Exploring that new approach, this study was carried out to assess the performance of a Platelia Dengue IgA Capture assay for dengue infection detection, newly developed by Bio-Rad, using 184 well-characterized samples provided by the French Guiana NRC sera collection of the Institut Pasteur in French Guiana. This study revealed good overall performances of this test, constituting promising assistance in dengue diagnosis, especially in hyper-endemic countries.
- Subjects
FRENCH Guiana; DENGUE hemorrhagic fever; DENGUE; IMMUNOGLOBULIN A; DENGUE viruses; CONVALESCENT plasma; VIRUS isolation
- Publication
PLoS Neglected Tropical Diseases, 2015, Vol 9, Issue 3, p1
- ISSN
1935-2727
- Publication type
Article
- DOI
10.1371/journal.pntd.0003596