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- Title
Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
- Authors
Yang, Ming-Chen; Wang, Chun-Chung; Tang, Wei-Chien; Chen, Kuan-Ming; Chen, Chu-Ying; Lin, Hsiao-Han; Hsieh, Yin-Cheng; Wang, Nan-Hsuan; Kuo, Yin-Chieh; Chu, Ping-Tzu; Tung, Hsin-Yi; Wu, Yi-Chen; Sun, Juo-Ling; Liu, Sheng-Yu; Li, Wan-Fen; Lee, Wei-Han; Lai, Jiann-Shiun; Chang, Michael; Lai, Ming-Tain
- Abstract
SARS-CoV-2 pandemic has profound impacts on human life and global economy since the outbreak in 2019. With the new variants continue to emerge with greater immune escaping capability, the protectivity of the available vaccines is compromised. Therefore, development a vaccine that is capable of inducing immunity against variants including omicron strains is in urgent need. In this study, we developed a protein-based vaccine BCVax that is consisted of antigen delta strain spike protein and QS21-based adjuvant AB801 in nanoparticle immune stimulation complex format (AB801-ISCOM). Results from animal studies showed that high level of anti-S protein IgG was induced after two doses of BCVax and the IgG was capable of neutralizing multiple variants of pseudovirus including omicron BA.1 or BA.2 strains. In addition, strong Th1 response was stimulated after BCVax immunization. Furthermore, BCvax with AB801-ISCOM as the adjuvant showed significant stronger immunity compared with the vaccine using aluminum hydroxide plus CpG 1018 as the adjuvant. BCVax was also evaluated as a booster after two prior vaccinations, the IgG titers and pseudovirus neutralization activities against BA.2 or BA.4/BA.5 were further enhanced suggesting BCVax is a promising candidate as booster. Taken together, the pre-clinical data warrant BCVax for further development in clinic.
- Subjects
SARS-CoV-2; IMMUNE response; COVID-19 vaccines; COVID-19 pandemic; SARS-CoV-2 Omicron variant
- Publication
PLoS ONE, 2023, Vol 17, Issue 3, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0283473