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- Title
miniSTR 基因座及其检测系统在降解检材中的法医学应用.
- Authors
王鑫; 陈维忠; 张健; 李景辉; 孙元鹏; 石云杰; 张雷; 陈林丽; 周翔; 周如华
- Abstract
Objective To establish multiplex system of 16 miniSTR loci, and explore its application value for the degraded materials in forensic medicine. Methods The multiplex system of 16 miniSTR loci was established using a six-dye fluorescence labeling technology and its application value in forensic medicine was assessed. Results A six-dye fluorescence labeling miniSTR amplification kit was developed, which enabled 15 autosomal STR loci, Amelogenin locus and DYS391 to be typed simultaneously. This method showed good specificity and could provide stable and accurate typing results with a sensitivity of 50 pg. This system also provided a good test result for the normal biological sample of actual cases. Conclusion The multiplex system of 16 miniSTR loci has application value for degraded and trace materials with the advantages of high sensitivity and database compatibility, which can be used for forensic casework.
- Publication
Journal of Forensic Medicine / Fayixue Zazhi, 2018, Vol 34, Issue 5, p532
- ISSN
1004-5619
- Publication type
Article
- DOI
10.12116/j.issn.1004-5619.2018.05.019