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- Title
Evaluation of PREVI Color Gram Automated Staining System on Positive Blood Culture Samples.
- Authors
Wong, River C. W.; Heung, Samuel S. Y.; Ho, Y. C.; Yip, K. T.; Que, T. L.
- Abstract
Objective: This study aims to evaluate the performance of PREVI Color Gram system among positive blood culture samples. Methods: A double-blind study was performed on gram smears prepared by the system using either commercial PREVI Color Gram dyes (n=157) or in-house homemade stains (n=167) and compared to the manual gram-stain method using subsequent culture identification as a reference standard. Results: The automatic method using PREVI Color Gram dyes and the manual method showed 99.2% (120/121) agreement with culture results (single organism). The concordant rate of automatic and manual methods with corresponding culture results (multiple organisms) were 96.3% (26/27) and 100% (27/27), respectively. The automatic method using an in-house homemade stain showed 99.3% (138/139) agreement with culture results (single organism), while the manual method was 98.6% (137/139). The automatic and manual methods gave a 91.7% (22/24) concordant rate with culture results (multiple organisms). Conclusions: PREVI Color Gram system can replace a conventional manual gram-stain method on clinical blood culture specimens.
- Subjects
STAINS &; staining (Microscopy); COMPARATIVE studies; COMPUTERS in medicine; MICROBIOLOGICAL techniques; QUALITY control; RESEARCH funding; SLIDES (Photography); BLIND experiment; MEDICAL equipment reliability; DESCRIPTIVE statistics; EQUIPMENT &; supplies
- Publication
Laboratory Medicine, 2011, Vol 42, Issue 7, p414
- ISSN
0007-5027
- Publication type
Article
- DOI
10.1309/LMZ5ZO7VW2WNKONX