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- Title
Preclinical in vitro study of streptococcus mutans accumulation in three fixed retainer designs: Microbiological assay.
- Authors
CURADO, Marcelo de Morais; MINERVINO, Bruno Lima; LEITE Jr., Cleiber de Oliveira; SALLES, Loise Pedrosa; de OLIVEIRA-SALLES, Gustavo Henrique; GANDINI Jr., Luiz Gonzaga; MACEDO, Sergio Bruzadelli
- Abstract
The use of fixed retainers in the lower arch is frequent; however, its presence increases the accumulation of biofilm and dental calculus. The objective of this research was to evaluate, in vitro, the accumulation of Streptococcus mutans (S. mutans) in 3 designs of fixed retainers. Nine models were reproduced in heat-cured acrylic resin and divided into groups: straight retainer (SR), retainer with vertical strap (RVS), retainer with horizontal strap (RHS). The accumulation of S. mutans was assessed using the MTT assay (3-4,5-dimethyl-thiazol-2-yl-2,5-diphenyltetrazolium bromide) and then measured using an automated reader. The RHS group showed less biofilm accumulation compared to the other groups (p<0.05). The distance between the tooth surface and the retainer showed a strong negative correlation with biofilm accumulation (rs=-0.79, p=0.00037). The RHS showed significantly less accumulation of S. mutans due to the distance between the retainer and the tooth surface. This research provides relevant data for a future randomized clinical trial.
- Subjects
STREPTOCOCCUS mutans; MICROBIOLOGICAL assay; DENTAL calculus; ORTHODONTIC retainers; CLINICAL trials; VACUUM packaging
- Publication
Dental Materials Journal, 2023, Vol 42, Issue 5, p646
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2023-003