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- Title
Effect of multiple copies of rpoBC on the rate of RNA synthesis in Escherichia coli.
- Authors
Guzman, Elena C.; Jimenez-Sanchez, Alfonso
- Abstract
The cloning of the rpoB and rpoC genes in a high copy number vector in E. coli increased the amount of the encoded gene products, the β and β′ subunits of RNA polymerase. However, this unexpectedly caused a 30–50% decrease in RNA synthetic activity which alternatively induced a reduction of growth rate and enlargement of cell size, and decreased the DNA replication time. The results can be explained by autogenous regulation of the RNA polymerase genes by the ββ′ subunits. A relation between the decrease in number of transcription units and the observed higher rate of movement of DNA replication forks is discussed.
- Publication
Genetics Research, 1986, Vol 48, Issue 2, p61
- ISSN
0016-6723
- Publication type
Article
- DOI
10.1017/S0016672300024770