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- Title
Intravenous immunoglobulin inhibits anti-glycoprotein IIb-induced platelet apoptosis in a murine model of immune thrombocytopenia.
- Authors
Leytin, Valery; Mykhaylov, Sergiy; Starkey, Alison F.; Allen, David J.; Lau, Herbert; Heyu Ni; Semple, John W.; Lazarus, Alan H.; Freedman, John
- Abstract
We have previously shown that injection of anti-glycoprotein (GP) IIb induces murine immune thrombocytopenia (ITP) and that intravenous immunoglobulin (IVIg) ameliorates ITP. We hypothesise that murine ITP may be associated with platelet apoptosis, which is upregulated by anti-GPIIb and downregulated by IVIg. The current study demonstrated that anti-GPIIb injection induced three critical apoptosis manifestations in platelets: (i) mitochondrial inner transmembrane potential (ΔΨm) depolarisation; (ii) caspase-3 activation; and (iii) phosphatidylserine (PS) exposure. IVIg administration inhibited caspase-3 activation and PS exposure, but not ΔΨm-depolarisation, in anti-GPIIb-treated platelets, demonstrating that IVIg ameliorates thrombocytopenia concomitantly with inhibiting late, but not early mechanisms of platelet apoptosis.
- Subjects
IMMUNOGLOBULINS; GLYCOPROTEINS; APOPTOSIS; BLOOD platelets; GLYCOPROTEIN hormones; THROMBOCYTOPENIA; PHOSPHATIDYLSERINES
- Publication
British Journal of Haematology, 2006, Vol 133, Issue 1, p78
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1111/j.1365-2141.2006.05981.x