We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Molecular characterization of hot spring cyanobacteria and evaluation of their photoprotective compounds.
- Authors
Rastogi, Rajesh P.; Kumari, Sunita; Richa; Han, Taejun; Sinha, Rajeshwar P.
- Abstract
Phylogenetic analysis of 4 cyanobacterial strains isolated from hot springs in Rajgir, India, was carried out using the 16S rRNA gene (1400 bp). These strains were identified as members of Chroococcales ( Cyanothece sp. strain HKAR-1) and Nostocales ( Nostoc sp. strain HKAR-2, Scytonema sp. strain HKAR-3, and Rivularia sp. strain HKAR-4). Furthermore, we evaluated the presence of ultraviolet-screening and (or) photoprotective compounds, such as mycosporine-like amino acids (MAAs) and scytonemin, in these cyanobacteria by using high-performance liquid chromatography. Well-characterized MAAs, including the critical and highly polar compounds shinorine, porphyra-334, and mycosporine-glycine, as well as several unknown MAAs, were found in these hot-spring-inhabiting microorganisms. The presence of scytonemin was detected only in Scytonema sp. strain HKAR-3 and Rivularia sp. strain HKAR-4. The results indicate that hot spring cyanobacteria, namely Cyanothece, Nostoc, Scytonema, and Rivularia, belonging to different groups possess various photoprotective compounds to cope up with the negative impacts of damaging radiations.
- Subjects
CYANOBACTERIA; PHYLOGENY; MYCOSPORINE-like amino acids; HIGH performance liquid chromatography; GLYCINE; POLYMERASE chain reaction
- Publication
Canadian Journal of Microbiology, 2012, Vol 58, Issue 6, p719
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/w2012-044