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- Title
The constant gene orf14.9, which belongs to the variable eps (exopolysaccharide) cluster, is involved in the cell growth of Streptococcus thermophilus.
- Authors
Tyvaert, Guillaume; Morel, Catherine; Joly, Jean-Pierre; Decaris, Bernard; Charron-Bourgoin, Florence
- Abstract
In Streptococcus thermophilus, the eps clusters involved in exopolysaccharide (EPS) biosynthesis are very polymorphic, nevertheless they all contain a highly conserved sequence corresponding to that of orf14.9. This open reading frame (ORF) is transcribed in a reverse direction with respect to eps genes. Amino acid sequence analysis showed a possible transmembrane location of the putative Orf14.9 protein but did not permit a proposed function. Insertional mutants of orf14.9 were obtained in strains NST2280 and A054 of S. thermophilus. EPS yields of these mutants are similar to those of their respective wild strains, suggesting that orf14.9 does not modify the quantity of produced EPS. Growth parameter determination for wild strains and their respective mutants showed that orf14.9 is involved in the cell growth of S. thermophilus.
- Subjects
STREPTOCOCCUS thermophilus; REGULATION of cell growth; AMINO acid sequence; BIOCHEMICAL engineering; PHYSIOLOGICAL control systems; CELLULAR control mechanisms; HEREDITY
- Publication
Canadian Journal of Microbiology, 2006, Vol 52, Issue 9, p908
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W06-047