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- Title
Identification of low-temperature-regulated genes in the fire blight pathogen Erwinia amylovora.
- Authors
Goyer, Claudia; Ullrich, Matthias S
- Abstract
Genes involved in pathogenicity of several plant pathogens were shown to be induced at relatively cold temperatures. Loci from the fire blight pathogen Erwinia amylovora (Burrill) induced at 18 °C were identified using the miniTn5 transposon that contains the promoterless reporter gene gusA coding for β-glucuronidase (GUS). Certain mutants (2.7%) expressed GUS predominantly at 18 °C on minimal medium plates, indicating that the transposon had been inserted downstream of a putatively thermoregulated promoter. Those mutants were further screened with a quantitative GUS fluorometric assay. A total of 21 mutants were selected: 19 mutants had a transposon insertion in temperature-dependent genetic loci, with a 2.2- to 6.3-fold induction of gusA gene expression at 18 °C, and two mutants with impaired growth at 18 °C. Some of these genetic loci encoded (i) proteins implicated in flagella biosynthesis, biotin biosynthesis, multi-drug efflux, and type II secretion protein, and (ii) proteins of unknown function.
- Subjects
PATHOGENIC microorganisms; MICROORGANISMS; ERWINIA amylovora; ERWINIA; GENES
- Publication
Canadian Journal of Microbiology, 2006, Vol 52, Issue 5, p468
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W05-153