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- Title
Xylanase Production by Aspergillus niger LPB 326 in Solid-State Fermentation Using Statistical Experimental Designs.
- Authors
Maciel, Giselle Maria; Vandenberghe, Luciana Porto de Souza; Haminiuk, Charles Windson Isidoro; Fendrich, Ricardo Cancio; Bianca, Bianca Eli Della; Brandalize, Tahiana Quintella da Silva; Pandey, Ashok; Soccol, Carlos Ricardo
- Abstract
Xylanase was produced by Aspergillus niger LPB 326 cultivated on lignocellulosic substrate composed by sugarcane bagasse and soybean meal in solid-state fermentation. The effects of various variables were observed and optimized by applying statistical experimental designs. The best xylanase activity was obtained in a medium containing 10 g of sugarcane bagasse and soybean meal in the ratio of 65 and 35 %, respectively, moistened to 85 % of initial water content with a nutrient salt solution composed of (in g/L): CuSO4 0.4, KH2PO4 1.5 and CoSO4 0.0012, and incubated for 4 days at 30 °C. Under these optimized conditions, a xylanase activity of 3099 IU/g of dry matter was obtained.
- Subjects
XYLANASES; ASPERGILLUS niger; SOLID-state fermentation; EXPERIMENTAL design; LIGNOCELLULOSE; BAGASSE
- Publication
Food Technology & Biotechnology, 2008, Vol 46, Issue 2, p183
- ISSN
1330-9862
- Publication type
Article