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- Title
Activation of Src-dependent Smad3 signaling mediates the neutrophilic inflammation and oxidative stress in hyperoxia-augmented ventilator-induced lung injury.
- Authors
Li-Fu Li; Chung-Shu Lee; Yung-Yang Liu; Chih-Hao Chang; Chang-Wei Lin; Li-Chung Chiu; Kuo-Chin Kao; Ning-Hung Chen; Cheng-Ta Yang; Li, Li-Fu; Lee, Chung-Shu; Liu, Yung-Yang; Chang, Chih-Hao; Lin, Chang-Wei; Chiu, Li-Chung; Kao, Kuo-Chin; Chen, Ning-Hung; Yang, Cheng-Ta
- Abstract
<bold>Background: </bold>Mechanical ventilation and concomitant administration of hyperoxia in patients with acute respiratory distress syndrome can damage the alveolar epithelial and capillary endothelial barrier by producing inflammatory cytokines and reactive oxygen species. The Src tyrosine kinase and Smad3 are crucial inflammatory regulators used for ventilator-induced lung injury (VILI). The mechanisms regulating interactions between high-tidal-volume mechanical ventilation, hyperoxia, and acute lung injury (ALI) are unclear. We hypothesized that high-tidal-volume mechanical stretches and hyperoxia augment lung inflammation through upregulation of the Src and Smad3 pathways.<bold>Methods: </bold>Wild-type or Src-deficient C57BL/6 mice, aged between 6 and 8 weeks, were exposed to high-tidal-volume (30 mL/kg) ventilation with room air or hyperoxia for 1-4 h after 2-mg/kg Smad3 inhibitor (SIS3) administration. Nonventilated mice were used as control subjects.<bold>Results: </bold>We observed that the addition of hyperoxia to high-tidal-volume mechanical ventilation further induced microvascular permeability, neutrophil infiltration, macrophage inflammatory protein-2 and matrix metalloproteinase-9 (MMP-9) production, malondialdehyde, nicotinamide adenine dinucleotide phosphate oxidase activity, MMP-9 mRNA expression, hypoxemia, and Src and Smad3 activation (P < 0.05). Hyperoxia-induced augmentation of VILI was attenuated in Src-deficient mice and mice with pharmacological inhibition of Smad3 activity by SIS3 (P < 0.05). Mechanical ventilation of Src-deficient mice with hyperoxia further reduced the activation of Smad3.<bold>Conclusions: </bold>Our data suggest that hyperoxia-increased high-tidal-volume ventilation-induced ALI partially depends on the Src and Smad3 pathways.
- Subjects
PNEUMONIA prevention; ANIMAL experimentation; ARTIFICIAL respiration; BIOLOGICAL models; CAPILLARY permeability; CARRIER proteins; CELLULAR signal transduction; CYTOKINES; DISEASE susceptibility; HETEROCYCLIC compounds; IMMUNITY; ISOQUINOLINE; LUNGS; LUNG injuries; MICE; NEUTROPHILS; OXIDOREDUCTASES; PNEUMONIA; PROTEOLYTIC enzymes; PYRIDINE; RESPIRATORY measurements; TRANSFERASES; PHENOTYPES; MECHANICAL ventilators; OXIDATIVE stress; HYPEROXIA; CHEMICAL inhibitors; DISEASE complications; PREVENTION
- Publication
Respiratory Research, 2015, Vol 16, Issue 1, p1
- ISSN
1465-9921
- Publication type
journal article
- DOI
10.1186/s12931-015-0275-6