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- Title
In Vitro and In Vivo Antitumor Effects of n-Butanol Extracts of Pterocephalus hookeri on Hep3B Cancer Cell.
- Authors
Chenxu Guo; Yingchun Wu; Yuanzhang Zhu; Yanchun Wang; Lili Tian; Yi Lu; Cong Han; Guofu Zhu
- Abstract
Pterocephalus hookeri is a widely applied Tibetan medicinal prescription for treatment of diseases such as flu, rheumatoid arthritis, and enteritis in China. It has been reported that Pterocephalus hookeri has anti-inflammatory and analgesic actions. However, the antitumor activity of Pterocephalus hookeri remains unknown. In the present study, we demonstrate that n-butanol extracts of Pterocephalus hookeri (YSC-ZDC) has a strong antitumor activity against hepatoma carcinoma cell in vitro and in vivo. YSC-ZDC inhibited proliferation of all cancer cell lines and significantly inhibited Hep3B cells proliferation in a dose- and time-dependant manner. Transmission electron microscopy, hoechst 33258 staining, and flow cytometry analysis revealed that YSC-ZDC induced apoptosis in Hep3B cells. YSC-ZDC treatment dramatically inhibited PDK1 and Akt phosphorylation in Hep3B cells. Moreover, YSC-ZDC increased Bax expression and inhibited Bcl-2 expression. In addition, YSC-ZDC inhibited growth hepatoma xenografts in vivo with no effect on body weight and spleen index. Consistent with results in vitro, YSC-ZDC increased Bax expression and inhibited Bcl-2 expression in tumor tissue. Taken together, this study shows YSC-ZDC with an antitumor activity both in vitro and in vivo. Its mechanism underlying is related to blocking of the Akt pathway and regulation of Bcl-2 family proteins expression.
- Subjects
CHINA; ACADEMIC medical centers; ANALYSIS of variance; APOPTOSIS; BIOPHYSICS; CELL culture; ELECTRON microscopy; FLOW cytometry; HEPATOCELLULAR carcinoma; HIGH performance liquid chromatography; IMMUNOHISTOCHEMISTRY; RESEARCH methodology; BOTANIC medicine; CHINESE medicine; POLYMERASE chain reaction; RESEARCH funding; T-test (Statistics); WESTERN immunoblotting; REVERSE transcriptase polymerase chain reaction; DATA analysis software; IN vitro studies; PREVENTION
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2015, p1
- ISSN
1741-427X
- Publication type
journal article
- DOI
10.1155/2015/159132