We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Nitric Oxide Synthesis Inhibition and Anti-Inflammatory Effect of Polypeptide Isolated from Chicken Feather Mea in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
- Authors
Aurachorn Inkanuwat; Romteera Sukaboon; Onrapak Reamtong; Pravit Asawanonda; Ake Pattaratanakun; Tanatorn Saisavoey; Papassara Sangtanoo; Aphichart Karnchanatat
- Abstract
Nitric oxide (NO) plays a key role in the pathogenesis of inflammation and has been implicated in endotoxin-induced tissue injury. Chicken feather meal is a rich source of amino acids that may serve as a peptide hydrolysate to inhibit NO activity. Anti-inflammatory hydrolysates of chicken feather meal were prepared and fractionated into five samples based on molecular mass. The smallest fraction (<0.65 kDa) exhibited the highest NO inhibitory activity without cytotoxicity towards macrophage RAW 264.7 cells. Further subfractions were sufficient to obtain amino acid sequences by Q-TOF LC-MS/MS ESI analysis. Of these, the SNPSVAGVR (885.97 Da) peptide and its corresponding pure synthetic peptide have inhibitory activity against NO production by RAW 264.7 cells (IC50=(55.2±0.2) mM) without cytotoxicity. Reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR results revealed that the peptide of the obtained fraction reduced transcript expression levels of the pro-inflammatory cytokines iNOS, TNF-a, COX-2 and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells. These results suggest that the peptides derived from the chicken feather meal protein could potentially be used as a promising ingredient in functional foods or nutraceuticals against inflammatory diseases.
- Subjects
REVERSE transcriptase polymerase chain reaction; NITRIC oxide; OXIDE synthesis; AMINO acid sequence; PEPTIDE antibiotics; FEATHERS
- Publication
Food Technology & Biotechnology, 2019, Vol 57, Issue 2, p200
- ISSN
1330-9862
- Publication type
Article
- DOI
10.17113/ftb.57.02.19.5964